J. Hiblot

Engineering of a biosensor for intracellular aspartate

The paper develops a biosensor, iAspSnFR, to measure intracellular aspartate levels in live cells and demonstrates its utility in studying metabolic pathways and cancer biology.

A general method for the development of multicolor biosensors with large dynamic ranges

The paper introduces a method to create multicolor biosensors with large dynamic ranges using FRET pairs based on fluorescent proteins and HaloTag.

Synergizing exchangeable fluorophore labels for multi-target STED microscopy

This paper demonstrates 6-target STED microscopy using exchangeable labels, improving multiplexing capabilities and reducing photobleaching in live-cell imaging.

Exchangeable HaloTag Ligands (xHTLs) for multi-modal super-resolution fluorescence microscopy

The paper introduces exchangeable HaloTag ligands for multi-modal super-resolution fluorescence microscopy, enabling dual-color imaging techniques like PAINT and STED.

Identification of a new Inovirus in the absence of bacterial infection in the human virome

The paper identifies a new Inovirus, RIP1, in the human virome without bacterial infection, showing its presence in multiple body sites.

Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes

The paper demonstrates that eight pairwise combinations of 18 synthetic fluorescent probes are suitable for fluorescence lifetime multiplexing in mammalian cells, enabling imaging of up to six biological targets.

HaloTag9: an engineered protein tag to improve fluorophore performance

HaloTag9 improves fluorophore performance with higher brightness and fluorescence lifetime, enabling live-cell multiplexing and biosensor generation.

Kinetic and structural characterization of the self-labeling protein tags HaloTag7, SNAP-tag and CLIP-tag

The paper compares the labeling kinetics and substrate specificities of HaloTag7, SNAP-tag, and CLIP-tag to guide their optimal use in bioimaging and biotechnology.