J. V. Rocheleau

Insulin secretion chip (InS-chip) reveals two peaks within first-phase that temporally coincide with a transition in glucose metabolism.

The paper introduces an islet-on-a-chip device (InS-chip) to measure individual islet insulin secretion with high temporal resolution, revealing two glucose-dependent peaks in first-phase secretion linked to metabolic transitions.

Targeting Apollo-NADP+ to image NADPH generation in pancreatic beta-cell organelles

The paper develops organelle-targeted Apollo-NADP+ sensors to study glucose- and glutamine-stimulated NADPH production in pancreatic beta-cell organelles, revealing mitochondria's key role in NADPH generation.

Islet-on-a-chip device reveals first phase glucose-stimulated respiration is substrate limited by glycolysis independent of Ca2+ activity.

The paper develops an islet-on-a-chip device to measure glucose-stimulated respiration and Ca2+ activity in individual islets, showing OxPhos is substrate-limited by NADH rather than ADP and independent of Ca2+.

Design of a Versatile Microfluidic Device for Imaging Precision-Cut-Tissue Slices

This paper designs a microfluidic device for 3D fluorescence imaging of precision-cut-tissue slices, enabling controlled media exchange and high-resolution imaging with confocal and SPIM.

Cell segmentation using deep learning: comparing label and label-free approaches using hyper-labeled image stacks

This study compares label and label-free deep learning approaches for cell segmentation using hyper-labeled image stacks, finding fluorescence-based methods are more accurate but label-free models can be more robust with inconsistent labeling.