J.-A. Daros

RNA virus-mediated gene editing for tomato trait breeding

This paper demonstrates the use of virus-induced genome editing (VIGE) in tomato to edit genes related to fruit color and achieve heritable trait modifications.

Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors

The paper describes a method to use RNA virus vectors for heritable CRISPR-Cas9 genome editing in plants through agroinoculation of sgRNA-containing viral constructs.

Conserved Structural Motifs in the Hammerhead Ribozyme of a Chloroplast Viroid Mimic tRNA Anticodon Structure to Hijack tRNA Ligase for Viroid Circularization

This paper shows that hammerhead ribozymes in viroids mimic tRNA anticodon loops to recruit tRNA ligase for circularizing replication intermediates.

CRISPR-Cas12a genome editing at the whole-plant level using two compatible RNA virus vectors

This paper demonstrates DNA-free genome editing in Nicotiana benthamiana using two compatible RNA virus vectors to deliver CRISPR-Cas12a components.

Carotenoid and tocopherol fortification of zucchini fruits using a viral RNA vector

This paper demonstrates a method to fortify zucchini fruits with carotenoids and tocopherols using a viral RNA vector without transgenic DNA.

Efficient Cas9 multiplex editing using unspaced gRNA arrays engineering in a Potato virus X vector

This paper develops a PVX-based vector for efficient multiplex CRISPR-Cas genome editing in plants by expressing arrays of unspaced gRNAs, achieving high indel rates in Nicotiana benthamiana.

Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system

This paper develops a virus-driven system in Nicotiana benthamiana to efficiently produce saffron crocins and picrocrocin in two weeks.