High-throughput, unbiased single-molecule displacement mapping with deep learning reveals spatiotemporal heterogeneities in intracellular diffusivity
Jiankai Xia, Yi He, Zhipeng Zhang, Jinhong Yan, Ruirong Wang, Ming Tang, J.C. Chen, Jun Fan, Kun Chen
This paper introduces Hi-SMdM, a high-throughput single-molecule diffusivity microscopy approach that enables unbiased mapping of spatiotemporal heterogeneities in intracellular diffusivity with improved resolution and throughput.
ACS Photonics · Apr 16, 2025Peer-reviewedBiophysics