Kun Chen

This paper introduces PhySMI, a self-supervised framework for multiplexed imaging without spectral measurements, achieving high multiplexing with reduced crosstalk and improved imaging performance.

This paper introduces LS-ExSM, a method for fast multiplexed imaging and quantitative physicochemical mapping in cells, demonstrating six-color volumetric imaging and lipid-droplet polarity mapping.

This paper introduces Hi-SMdM, a high-throughput single-molecule diffusivity microscopy approach that enables unbiased mapping of spatiotemporal heterogeneities in intracellular diffusivity with improved resolution and throughput.

ExSPM reveals spatiotemporal heterogeneities in lipid droplet polarity, showing dynamics and pathway-specific kinetics of lipid metabolism.

This study introduces pc-SMdM, a super-resolution technique to map nanoscale diffusivity on cellular membranes, revealing clusters influenced by cholesterol and membrane structure.