CryoSHIELDs: tunable amphipathic peptide additives for cryo-EM
Caleigh M. Azumaya, Przemysław Dutka, Matthew Camp Johnson, Cheng-Guo Wu, Bryce D. Delgado, Christopher W. Koo, Jeffrey Tom, Tik Hang Soong, Sandeep Kaur Ravala, Alister Burt, Shu Ti, Anna C. Howes, Orlando E. Martinez, Aimin Song, Alexis L. Rohou, , Stanislau Yatskevich
This paper introduces cryoSHIELDs, tunable peptides that improve cryo-EM sample preparation by modulating the air-water interface, enhancing particle distribution and complex integrity across multiple protein systems.
bioRxiv · Oct 8, 2026 · PreprintBiophysics