Alister Burt

CryoSHIELDs: tunable amphipathic peptide additives for cryo-EM

This paper introduces cryoSHIELDs, tunable peptides that improve cryo-EM sample preparation by modulating the air-water interface, enhancing particle distribution and complex integrity across multiple protein systems.

MemBrain v2: an end-to-end tool for the analysis of membranes in cryo-electron tomography

MemBrain v2 couples a generalist U-Net cryo-ET membrane segmenter, a mesh-based picker reaching 91% F1 for photosystem II from one annotated membrane, and geodesic particle statistics.

Easymode: general pretrained networks for cellular cryo-ET enable flexible approaches to subtomogram averaging

Easymode provides pretrained networks for cryo-ET segmentation, enabling efficient subtomogram averaging and cellular context analysis without per-dataset training.

MissAlignment Teaches Itself Better Cryo-ET Tilt-Series Alignment by Making It Worse

MissAlignment improves cryo-ET tilt-series alignment by training a neural network to recognize misalignment without requiring well-aligned ground truth.

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells

The paper determines the sub-nanometer structure of the SUN5-Nesprin3 LINC complex in human sperm cells, revealing its role in nuclear envelope remodelling and male infertility.

Human spermatogenesis leads to a reduced nuclear pore structure and function

This study investigates the structural and functional changes in human sperm nuclear pore complexes (NPCs) during spermatogenesis, revealing a reduced central channel and altered transport dynamics.

An image processing pipeline for electron cryo-tomography in RELION-5

The paper introduces an image processing pipeline for electron cryo-tomography in RELION-5, enabling automated atomic model building from tomographic data.

Mechanism of outer kinetochore assembly on microtubules and its regulation by mitotic error correction

The paper describes the structure of the budding yeast outer kinetochore complexes and how phosphorylation regulates their stability during mitotic error correction.

A robust normalized local filter to estimate compositionalheterogeneity directly from cryo-EM maps

This paper introduces a normalized local filter to estimate compositional heterogeneity in cryo-EM maps and demonstrates its utility for interpretation and signal subtraction.