A Strategy to Assess the Cellular Activity of E3 Ligases against Neo-Substrates using Electrophilic Probes
Benika J. Pinch, Dennis L. Buckley, Scott Gleim, Scott M. Brittain, Laura Tandeske, Pier Luca D’Alessandro, Zachary J. Hauseman, Jennifer Lipps, Lei Xu, Edward P. Harvey, Markus Schirle, Elizabeth R. Sprague, William C. Forrester, Dustin Dovala, Lynn M. McGregor, Claudio R. Thoma
This paper introduces a method to assess E3 ligase activity against neo-substrates using electrophilic probes, demonstrating its effectiveness with VHL and SPSB2.
Cell chemical biology · Sep 8, 2021Peer-reviewedMolecular Biology