CRISPR/Cas9-based mutagenesis frequently provokes on-target mRNA misregulation
Rubina Tuladhar, Yunku Yeu, John Tyler Piazza, Zhen Tan, Jean Rene V. Clemenceau, Xiaofeng Wu, Quinn Barrett, Jeremiah Herbert, David H. Mathews, James Kim, Tae Hyun Hwang, Lawrence Lum
This study shows that CRISPR/Cas9-induced INDELs can lead to mRNA misregulation by creating foreign mRNAs or proteins in about half of edited cell lines.
Nature Communications · Sep 6, 2019Peer-reviewedMolecular Biology