BiophysicsPLoS ONEPeer-reviewed
Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure
Anne R. Shim, Jane Frederick, Emily M. Pujadas-Liwag…·Vadim Backman
This study shows that formamide in 3D FISH distorts nanoscale chromatin structure, while alternative methods like RASER-FISH and CRISPR-Sirius have minimal impact.
14 citations