Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociation
Jinglong Wang, Julien Le Gall, Richard L. Frock, Terence R. Strick
The paper shows that shifting the RNA:DNA hybrid relative to the PAM in SpCas9 generates DNA overhangs and accelerates complex dissociation after cleavage.
Nature Structural & Molecular Biology · Oct 12, 2023Peer-reviewedGenomics