The establishment of multiple knockout mutants of Colletotrichum orbiculare by CRISPR/Cas9 and Cre/loxP systems
K. Yamada, T. Yamamoto, K. Uwasa, K. Osakabe, Y. Takano
This paper develops CRISPR/Cas9 and Cre/loxP systems to efficiently generate multiple knockout mutants in Colletotrichum orbiculare and identifies functional sugar transporters.
bioRxiv · Oct 24, 2021Plant Biology