High-resolution fleezers reveal duplex opening and stepwise assembly by an oligomer of the DEAD-box helicase Ded1p
Eric M. Patrick, Rajeev Yadav, Kasun B. Senanayake, Kyle Cotter, Andrea A. Putnam, Eckhard Jankowsky, Matthew Comstock
This study uses high-resolution optical tweezers to show Ded1p oligomers unwind RNA duplexes in 4-6 bp steps through dynamic protomer assembly and ATP hydrolysis.
Nature Communications · Jan 25, 2025Peer-reviewedBiophysics