Andrea A. Putnam

Molecular and cellular characterization of the interaction between the Rho GAP RGA-3/4 and F-actin

This study investigates how RGA-3/4 regulates F-actin structure in vivo and in vitro, showing it directly binds and bundles F-actin, providing insights into its role in cortical excitability and cytokinesis.

RNA-dependent aggregation of a common TEV protease variant alters in vitro biomolecular condensate reconstitution

The paper shows that TEV protease variants can aggregate with RNA, altering in vitro biomolecular condensate formation and highlights the need for validation in RNA-rich systems.

Phase separation of PGL-3 driven by structured domains that oligomerize and interact with terminal RGG motifs.

This study identifies that structured domains in PGL-3 drive phase separation through oligomerization and interaction with RGG motifs, independent of RNA.

High-resolution fleezers reveal duplex opening and stepwise assembly by an oligomer of the DEAD-box helicase Ded1p

This study uses high-resolution optical tweezers to show Ded1p oligomers unwind RNA duplexes in 4-6 bp steps through dynamic protomer assembly and ATP hydrolysis.

Pickering stabilization of a dynamic intracellular emulsion

The paper shows that interfacial protein clusters stabilize biomolecular condensates by regulating their dynamics and structural integrity.

Analysis of P granules in vivo and ex vivo

The paper analyzes the material properties of P granules in vivo and ex vivo, finding a non-dynamic shell that stabilizes their liquid phase.