Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces
Andrea Ameruoso, Maria Claudia Villegas Kcam, Katherine Piper Cohen, James Chappell
This paper develops CRISPRi and CRISPRa systems in Streptomyces to activate silent biosynthetic gene clusters for antibiotic discovery.
Nucleic Acids Research · Jul 8, 2022Peer-reviewedSynthetic Biology