Author

Keiko Masuda

RIKEN Center for Biosystems Dynamics Research

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Synthetic Biology· 3 Biochemistry· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Organism:bacteria (other)· 1 Assay:protein structure· 1 Assay:proteomics· 1 Research Focus:synaptic & neural circuits· 1 Research Focus:variant calling· 1

Frequent Co-Authors

Yoshihiro ShimizuiD· 4 shared Norikazu IchihashiiD· 2 shared Aya SatoiD· 1 shared Katsumi HaginoiD· 1 shared Keiko Kasahara· 1 shared Masaru Shimojo· 1 shared Ryohei NarumiiD· 1 shared Ryota MiyachiiD· 1 shared

Activity by Year

2021-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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4 shared papersSynthetic Biology

Yoshihiro ShimizuORCID

Mutual co-authors in cluster

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4 papers

Recent·Most cited

Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method

Ryota Miyachi, Keiko Masuda, Yoshihiro Shimizu, Norikazu Ichihashi

This study develops a tRNA array method to simultaneously express all 21 tRNAs in vitro, supporting translation and genetic code engineering.

Nature Communications · Aug 26, 2025Peer-reviewedSynthetic Biology

Sustainable Regeneration of 20 Aminoacyl-tRNA Synthetases in a Reconstituted System Toward Self-Synthesizing Artificial Systems

Katsumi Hagino, Keiko Masuda, Yoshihiro Shimizu, Norikazu Ichihashi

The paper develops a reconstituted system that sustainably regenerates all 20 aminoacyl-tRNA synthetases for up to 20 cycles.

Science Advances · Apr 2, 2025Peer-reviewedSynthetic Biology

Single-step in vitro ribosome reconstitution mediated by two GTPase factors, EngA and ObgE

Aya Sato, Weng Yu Lai, Yusuke Sakai, Keiko Masuda, Yoshihiro Shimizu

This study develops a single-step in vitro ribosome reconstitution method using EngA and ObgE to restore ribosome activity under near-physiological conditions.

bioRxiv · Oct 17, 2024 · PreprintBiochemistry

Versatile and multiplexed mass spectrometry-based absolute quantification with cell-free-synthesized internal standard peptides

Keiko Masuda, Keiko Kasahara, Ryohei Narumi, Masaru Shimojo, Yoshihiro Shimizu

The paper introduces an improved method for multiplexed absolute protein quantification using mass spectrometry with cell-free synthesized internal standard peptides.

Journal of Proteomics · Oct 22, 2021Peer-reviewedSynthetic Biology