Ryota Miyachi

Experimental verification of the error minimization theory using non-standard genetic codes constructed in vitro

The paper constructs non-standard genetic codes in vitro and shows that mutational robustness of reporter proteins remains similar despite changes in genetic code, supporting the error minimization theory.

Enhanced tRNA array method version 2 for simultaneous in vitro synthesis of 21 tRNAs

The paper improves the tRNA array method to enhance translational activity for multiple reporter proteins under various conditions.

Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method

This study develops a tRNA array method to simultaneously express all 21 tRNAs in vitro, supporting translation and genetic code engineering.