Long-term co-maturation of stem cell-derived microglia and neuronal networks: an optimized platform to assess human microglial contribution to neuronal function
Annika Mordelt, Imke M. E. Schuurmans, Nicky Scheefhals, Marina P. Hommersom, Koen Slottje, Kimberly Mast, Mara Graziani, Carlos O. González, Laura J. A. Wingens, Dirk Schubert, Nael Nadif Kasri, Lot D. de Witte
This paper develops a co-maturation protocol to study human microglia-neuron interactions using hiPSCs, showing astrocytes are needed for microglia survival and integration into neuronal networks.
bioRxiv · Oct 24, 2025 · PreprintNeuroscience