Nicky Scheefhals

Navigating Human Astrocyte Differentiation: Direct and Rapid one-step Differentiation of Induced Pluripotent Stem Cells to Functional Astrocytes Supporting Neuronal Network development

This study presents a one-step protocol to differentiate hiPSCs into functional astrocytes, enabling co-culture with neurons to support network development.

MEA-LINK identifies the CCL4-CCR5 axis in neuronal hyperactivity control by human microglia

MEA-LINK identifies CCL4-CCR5 signaling as a mechanism by which human microglia regulate neuronal hyperactivity through secreted factors and network activity.

Long-term co-maturation of stem cell-derived microglia and neuronal networks: an optimized platform to assess human microglial contribution to neuronal function

This paper develops a co-maturation protocol to study human microglia-neuron interactions using hiPSCs, showing astrocytes are needed for microglia survival and integration into neuronal networks.

Human-Specific Regulation of CHD2 Controls the Tempo of Cortical Development through Lysosomal Homeostasis

This study identifies a human-specific CHD2 regulatory variant that influences cortical development tempo via lysosomal homeostasis, showing therapeutic potential through lysosomal restoration.

SCN1A-deficient hiPSC-derived excitatory neuronal networks display mutation-specific phenotypes

This study investigates SCN1A mutations in excitatory neuronal networks derived from patient hiPSCs, revealing mutation-specific phenotypes that correlate with clinical outcomes and drug responses.

ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons

ORANGE enables targeted genomic integration of fluorescent tags in neurons to study endogenous protein distribution with high-resolution imaging.