Expression, Purification, and Functional Reconstitution of 19F-labeled Cytochrome b5 in Peptide Nanodiscs for NMR Studies
J. Bai, J. Wang, T. R. Ravula, S.-C. Im, G. M. Anantharamaiah, L. Waskell, A. Ramamoorthy
This study expresses, purifies, and functionally reconstitutes 19F-labeled cytochrome b5 in peptide nanodiscs to enable NMR studies of its transmembrane domain interactions with CYP2B4.
bioRxiv · Nov 16, 2019Biochemistry