A. Ramamoorthy

Nanodisc reconstitution and characterization of amyloid-β precursor protein C99

This study reconstitutes and characterizes the C99 domain of APP in nanodiscs using multiple biophysical methods.

On-Pathway Oligomer of Human Islet Amyloid Polypeptide Induced and Stabilized by Mechanical Rotation During MAS NMR

This study produces stable, on-pathway oligomers of hIAPP using mechanical rotation during MAS NMR, showing sensitivity to sample conditions and potential for studying other aggregating systems.

Factors influencing the detergent-free membrane protein isolation using synthetic nanodisc-forming polymers

This study evaluates the efficacy of inulin-based polymers in solubilizing E. coli membranes containing specific membrane proteins under various conditions.

Non-micellar ganglioside GM1 induces an instantaneous conformational change in Aβ42 leading to the modulation of the peptide amyloid-fibril pathway

This study shows non-micellar GM1 induces a conformational change in Aβ42, modulating its fibril formation pathway and exhibiting prion-like properties.

Ganglioside GM1 produces stable, short, and cytotoxic Aβ40 protofibrils

This study shows that GM1 stabilizes short, cytotoxic Aβ40 protofibrils and enhances aggregation of both Aβ40 and Aβ42.

Synthesis, Characterization, and Nanodisc formation of Non-ionic Polymers

This paper reports non-ionic amphiphilic polymers that form stable lipid-nanodiscs and efficiently extract membrane proteins.

Natural-abundance 17O NMR spectroscopy of magnetically aligned lipid nanodiscs

The paper uses natural-abundance 17O NMR to study water ordering in magnetically aligned lipid nanodiscs, combining RQCs and molecular dynamics simulations.

Degradation of Alzheimers Amyloid-beta by a Catalytically Inactive Insulin Degrading Enzyme

This study shows that a catalytically inactive insulin-degrading enzyme variant can degrade Alzheimer's amyloid-beta via a non-chaperone mechanism, with zinc binding inactivating its function.

Conformational Tuning of Amylin by Charged Styrene-maleic-acid Copolymers

This study shows that charged styrene-maleic-acid copolymers alter amylin aggregation and conformation, with SMAQA inhibiting fibrillation and SMAEA accelerating it.

Small Molecule Induced Toxic Human-IAPP Species Characterized by NMR

This study shows CurDAc inhibits hIAPP aggregation and disaggregates fibers, producing a toxic oligomer.

High Throughput Screening at the Membrane Interface Reveals New Inhibitors of Amyloid-β

This study identifies 5 small molecules that inhibit amyloid-β aggregation at the cell-membrane interface, which is relevant for Alzheimer's disease.

Expression, Purification, and Functional Reconstitution of 19F-labeled Cytochrome b5 in Peptide Nanodiscs for NMR Studies

This study expresses, purifies, and functionally reconstitutes 19F-labeled cytochrome b5 in peptide nanodiscs to enable NMR studies of its transmembrane domain interactions with CYP2B4.

Magnetic-Alignment of Polymer Nanodiscs Probed by Solid-State NMR Spectroscopy

This study investigates the magnetic alignment of SMA-QA polymer nanodiscs using solid-state NMR, showing their orientation changes in response to magnetic fields and paramagnetic salts.

Apolipoprotein A-I Mimetic 4F Peptide Generates Amyloid Cytotoxins by Forming Hetero-oligomers with β-amyloid

The paper investigates how the 4F peptide interacts with β-amyloid to form toxic heteromers, reducing aggregation kinetics and increasing neurotoxicity.

Probing protein-protein and protein-substrate interactions in the dynamic membrane-associated ternary complex of Cytochromes P450, b5, and Reductase

The paper uses NMR to study dynamic interactions in a ternary complex of Cytochromes P450, b5, and Reductase within lipid nanodiscs with and without substrates.

Exploiting Heterogeneous Time Scale of Dynamics toEnhance 2D HETCOR Solid-State NMR Sensitivity

This paper introduces two 2D HETCOR NMR pulse sequences to enhance sensitivity for rigid and mobile components in heterogeneous systems by leveraging different MAS conditions and polarization transfer mechanisms.

Magnetic-alignment of polymer macro-nanodiscs enable residual dipolar couplings based high-resolution structural studies by NMR

This paper shows that polymer macro-nanodiscs can be aligned magnetically to measure residual dipolar couplings (RDCs) using high-resolution NMR for structural studies.

Self-assembly of polymer-encased lipid nanodiscs and membrane protein reconstitution

This study optimizes coarse-graining models for polymer-encapsulated lipid nanodiscs to better study membrane protein structure and function.

Alzheimer’s Amyloid-Beta Intermediates Generated by Polymer-Nanodiscs

This study uses PMA-nanodiscs to study Aβ1-40 aggregation, showing they trap intermediates and reduce neurotoxicity of oligomers.

Cationic polymethacrylate-copolymer acts as an agonist for beta-amyloid and antagonist for amylin fibrillation

This study shows a cationic polymer promotes Aβ40 fibrillation and inhibits hIAPP aggregation through electrostatic interactions and structural restraint.

Zinc Boosts EGCG’s hIAPP Amyloid Inhibition Both in Solution and Membrane

This study shows that zinc enhances EGCG's ability to inhibit hIAPP amyloid aggregation both in solution and in the presence of lipid membranes.