Single Tube qPCR detection and quantitation of hotspot mutations down to 0.01% VAF
K. Zhang, L. Rodriguez, L. Y. Cheng, D. Y. Zhang
This paper introduces As-BDA, a method that detects and quantifies rare mutations down to 0.01% VAF using qPCR, with high concordance compared to ddPCR.
bioRxiv · Aug 12, 2021 · PreprintBioengineering