Pathway elucidation of pharmaceutical cucurbitacin IIa in Hemsleya chinensis and high-level production of precursor cucurbitadienol in engineered Saccharomyces cerevisiae and Nicotiana benthamiana
Geng Chen, Zhaokuan Guo, Yan Zhao, Yanyu Shu, Lei Qiu, Shao-feng Duan, Yuan Lin, Simei He, Xiaobo Li, Xiao‐Lin Feng, Guisheng Xiang, Yang Shi, Sheng-Chao Yang, Guanghui Zhang, Bing Hao
This study elucidates the biosynthetic pathway of cucurbitacin IIa in Hemsleya chinensis and demonstrates high-level production of its precursor in yeast and tobacco.
bioRxiv · Sep 28, 2022 · PreprintSynthetic Biology