2D, or not 2D? Investigating Vertical Signal Integrity of Tissue Slices
Sebastian Tiesmeyer, Niklas Müller‐Bötticher, Alexander Malt, Leyao Ma, Sergio Marco Salas, Paul Kießling, Paul Horn, Adrien Guillot, Louis Benedikt Kuemmerle, Frank Tacke, Fabian Joachim Theis, Christoph Kuppe, Mats F. Nilsson, Roland Eils, Brian Long, Naveed Ishaque
This paper introduces a tool to detect overlapping cells and segmentation errors in 3D spatial transcriptomics data.
Nature Biotechnology · Feb 10, 2026Peer-reviewedBioinformatics