Electrophile scanning by chemical proteomics reveals a potent pan-active DUB probe for investigation of deubiquitinase activity in live cells
Daniel Conole, Fangyuan Cao, Christopher W. am Ende, Liang Xue, Sheila Kantesaria, Dahye Kang, Jun Hong Jin, Dafydd R. Owen, Linda Lohr, Monica Schenone, Jaimeen D. Majmudar, Edward W. Tate
The paper identifies a small molecule DUB probe, IMP-2373, that quantitatively engages multiple DUBs in live cells and demonstrates its use in studying DUB activity changes during MYC deregulation.
bioRxiv · Sep 28, 2022 · PreprintSystems Biology