Partner-independent fusion gene detection by multiplexed CRISPR/Cas9 enrichment and long-read Nanopore sequencing
Christina Stangl, Sam de Blank, Ivo Renkens, Liset Westera, Tamara C. A. I. Verbeek, Jose Espejo Valle-Inclán, Rocío Chamorro González, Anton George Henssen, Markus J. van Roosmalen, Ronald W. Stam, Emile E. Voest, Wigard P. Kloosterman, Gijs W van Haaften, Glen R. Monroe
This paper introduces FUDGE, a method for detecting fusion genes using CRISPR/Cas9 enrichment and long-read sequencing, achieving high sensitivity and specificity across various cancer types.
Nature Communications · Jun 5, 2020Peer-reviewedGenomics