Barcoded oligonucleotides ligated on RNA amplified for multiplex and parallel in-situ analyses
Eswar P. R. Iyer, Sukanya Punthambaker, Songlei Liu, Kunal Jindal, Michael Farrell, Jernej Murn, Thomas Ferrante, Stewart Rudnicki, Richie E. Kohman, Asmamaw T. Wassie, Daniel Goodwin, Fei Chen, Shahar Alon, Anubhav Sinha, Denitsa Milanova, , George M. Church
The paper introduces BOLORAMIS, a RT-free method for spatially-resolved, targeted in-situ RNA detection, and demonstrates its use on human iPSCs to identify spatial localization and expression heterogeneity.
bioRxiv · Mar 20, 2018 · PreprintCell Biology