CTCF regulates wild-type and recombinant AAV gene expression by shaping viral chromatin
Clairine I. S. Larsen, Rhiannon R. Abrahams, Rea Guertler, Elliott M. Wilion, Eda Erata, Zachary H. Sykes, Lorelei Stoica, Gopishankar Thirumoorthy, Divya Sinha, Richa Rai, Elizabeth K. Hofer, Eleanor Hart, David M. Gamm, Matthew S. Fuller, Kinjal Majumder
This study identifies a CTCF-binding element in the wtAAV2 genome that enhances rAAV transgene expression by shaping chromatin structure, offering a strategy to improve gene therapy vectors.
bioRxiv · Jun 3, 2026 · PreprintMicrobiology