In vitro synthesis and reconstitution using mammalian cell-free lysates enables the systematic study of the regulation of LINC complex assembly
S. Majumder, Y.-Y. Hsu, H. Moghimianavval, M. Andreas, T. W. Giessen, G. W. G. Luxton, A. P. Liu
This paper develops a cell-free system to study LINC complex assembly by reconstituting membrane proteins in lipid bilayers and shows its utility in investigating chemical and molecular factors affecting protein interactions.
bioRxiv · Apr 11, 2021Biochemistry