T. W. Giessen

Structural and biochemical characterization of an encapsulin-associated rhodanesefrom Acinetobacter baumannii

This study characterizes the structure and biochemical function of an Acinetobacter baumannii RLD, showing its sulfurtransferase activity and potential ternary catalytic mechanism.

Point mutation in a virus-like capsid drives symmetry reduction to form tetrahedral cages

The paper shows that a single point mutation in a virus-like capsid can drive the formation of tetrahedral structures instead of the typical icosahedral symmetry.

Cyclodipeptide oxidase is an enzyme filament

The paper reports the structural characterization of the cyclodipeptide oxidase AlbAB from Streptomyces noursei, revealing it as a filamentous enzyme essential for antibiotic biosynthesis.

Structural basis for peroxidase encapsulation in a protein nanocompartment

This study determines the structural basis for encapsulating dye-decolorizing peroxidases in a protein nanocompartment using cryo-EM and mutagenesis.

HIV-1 binds dynein directly to hijack microtubule transport machinery

This study shows HIV-1 directly binds dynein to hijack microtubule transport for motility, bypassing traditional cargo adaptors.

Structure and heterogeneity of a highly cargo-loaded encapsulin shell

The paper reports the cryo-EM structure of a highly cargo-loaded encapsulin shell and identifies structural distortions caused by high cargo loading.

A widespread proteinaceous sulfur storage compartment in bacteria

The paper identifies desulfurase encapsulins as a novel sulfur storage compartment in bacteria, showing they sequester elemental sulfur and enhance desulfurase activity.

Exploring the Extreme Acid Tolerance of a Dynamic Protein Nanocage

This study characterizes AaEnc, an acid-stable encapsulin nanocage from Acidipropionibacterium acidipropionici, revealing its pH-dependent dynamic pore and extreme acid tolerance.

In vitro synthesis and reconstitution using mammalian cell-free lysates enables the systematic study of the regulation of LINC complex assembly

This paper develops a cell-free system to study LINC complex assembly by reconstituting membrane proteins in lipid bilayers and shows its utility in investigating chemical and molecular factors affecting protein interactions.

Large-scale computational discovery and analysis of virus-derived microbial nanocompartments

The paper identifies and analyzes over 6,000 encapsulin-like systems across bacterial and archaeal phyla, revealing novel families and operons, and proposes evolutionary links to HK97-type bacteriophages.

Structure and function of a 9.6 megadalton bacterial iron storage compartment

This study characterizes a bacterial iron storage compartment using structural methods, revealing its large iron storage capacity and functional mechanism.