In vitro Evolution of Antibody Affinity via Insertional Mutagenesis Scanning of an Entire Antibody Variable Region
K. Skamaki, S. Emond, M. Chodorge, J. Andrews, D. G. Rees, D. Cannon, B. Popovic, A. Buchanan, R. Minter, F. Hollfelder
This paper demonstrates that insertional mutagenesis can enhance antibody affinity, achieving a 256-fold improvement in an anti-IL-13 antibody through InDel mutagenesis combined with point mutations.
bioRxiv · Apr 26, 2020Biochemistry