M. D. Lynch

Precipitation and Extraction Methods for Protein Purification: A Meta-Analysis of Purification Performance and Cost-Effectiveness

This study reviews 168 publications on protein precipitation and liquid-liquid extraction, finding these methods are not always cheaper than chromatography and require optimization for cost-effectiveness.

Multi-scale spatial mapping of cell populations across anatomical sites in healthy human skin and basal cell carcinoma

The paper creates a multi-scale spatial atlas of healthy human skin and basal cell carcinoma using various omics and imaging techniques, revealing cell population localization and origins.

Optimization of Phosphate Limited Autoinduction Broth for 2-Stage Heterologous Protein Expression in E. coli

This paper develops a simplified autoinduction broth (AB-2) for 2-stage protein expression in E. coli, showing it is robust and performs as well as existing methods across different scales.

BioSamplr: An open source, low cost automated sampling system for bioreactors.

BioSamplr is an open-source, low-cost automated bioreactor sampling system that improves reproducibility and reduces hands-on time by enabling 24/7 sampling.

Dynamic control over feedback regulatory mechanisms improves NADPH fluxes and xylitol biosynthesis in engineered E. coli

This study improves NADPH flux and xylitol production in engineered E. coli by dynamically controlling regulatory metabolites and metabolic pathways.

Dynamic control over feedback regulation identifies pyruvate-ferredoxin oxidoreductase as a central metabolic enzyme in stationary phase E. coli.

This study uses dynamic metabolic control to enhance stationary phase biosynthesis in E. coli by targeting citrate synthase and glucose-6-phosphate dehydrogenase, leading to increased acetyl-CoA production and citramalic acid titers.

CRISPR/Cas "non-target" sites inhibit on-target cutting rates

This study shows that non-target PAM sites inhibit Cas9/gRNA on-target activity in a dose-dependent manner, suggesting strategies to improve Cas9 specificity.

Accounting for Gene Flow from Unsampled `Ghost' Populations while Estimating Evolutionary History under the Isolation with Migration Model

This paper evaluates biases in estimating evolutionary history when gene flow from unsampled ghost populations is not accounted for, using simulations and empirical data.