Quantitative crosslinking and mass spectrometry determine binding interfaces and affinities mediating kinetochore stabilization
G. Hagemann, V. Solis-Mezarino, S. Singh, M. Potocnjak, C. Kumar, F. Herzog
This study uses quantitative XLMS to determine binding interfaces and affinities in the kinetochore complex, showing phosphorylation reduces KD values by ~200-fold, essential for cell viability.
bioRxiv · Mar 31, 2022 · PreprintBiochemistry