A. Akhmanova

A network of interacting ciliary tip proteins with opposing activities imparts slow and processive microtubule growth

The paper investigates the interactions of ciliary tip proteins and their role in microtubule dynamics, finding that they form structures that slow and processively elongate microtubules.

GelMap: Intrinsic calibration and deformation mapping for expansion microscopy

GelMap introduces a fluorescent grid to measure expansion factors and deformation in expansion microscopy, enabling precise quality control and sample navigation.

CAMSAP-driven microtubule release from γ-TuRC and its regulation by nucleation-promoting factors

This study investigates how CAMSAP proteins and CDK5RAP2 regulate microtubule release from γ-TuRC, revealing their roles in microtubule nucleation and stabilization.

CSPP1 stabilizes growing microtubule ends and damaged lattices from the luminal side.

This study identifies CSPP1 as a microtubule stabilizer that binds to growing and damaged microtubules, providing insights into its role in ciliary and centriolar structure maintenance.

Estimation of microtubule-generated forces using a DNA origami nanospring

This paper introduces a DNA origami nanospring to estimate microtubule-generated forces using fluorescence microscopy and validates it against optical trapping.

Compressive forces stabilise microtubules in living cells

The paper shows that compressive forces stabilize microtubules in living cells through CLASP2 relocation, indicating mechano-responsive properties.

Organization and dynamics of the cortical complexes controlling insulin secretion in β-cells.

The paper investigates cortical complexes in β-cells that regulate insulin secretion, identifying non-neuronal proteins and showing their role in organizing secretory machinery at integrin-based adhesions.

Microtubule plus-end regulation by centriolar cap proteins

The paper investigates how CP110 and CEP97 regulate microtubule plus-end dynamics and centriole biogenesis through direct interactions and synergistic effects.

Opto-Katanin: An Optogenetic Tool for Localized Microtubule Disassembly

This paper introduces opto-katanin, a light-activated tool for localized microtubule disassembly, enabling precise spatiotemporal control in mammalian cells.

Angiomotin isoform 2 promotes binding of PALS1 to KIF13B at the base of primary cilia and suppresses ciliary elongation

This study identifies Angiomotin isoform 2 as a KIF13B interactor that recruits PALS1 to the base of primary cilia, regulating ciliary length by modulating KIF13B cargo binding.

Self-assembly of pericentriolar material in interphase cells lacking centrioles

This study shows that PCM can self-assemble into a compact MTOC in interphase cells without centrioles, using dynein transport and self-clustering, but this process is sensitive to motor and microtubule dynamics.

Optical nanoscopy reveals SARS-CoV-2-induced remodeling of human airway cells

This study uses advanced microscopy to show how SARS-CoV-2 changes the structure of human airway cells at the subcellular level.

Cross-linkers at growing microtubule ends generate forces that drive actin transport

This paper shows that passive cross-linkers between actin and growing microtubules generate forces that transport actin filaments, revealing a new mechanism for actin remodeling.

Photoswitchable epothilone-based microtubule stabilisers allow GFP imaging-compatible, optical control over the microtubule cytoskeleton

This paper introduces photoswitchable epothilone reagents (STEpos) that allow optical control over microtubule stability and dynamics with high spatiotemporal precision.

In vivo photocontrol of microtubule dynamics and integrity, migration and mitosis, by the potent GFP-imaging-compatible photoswitchable reagents SBTubA4P and SBTub2M

This paper introduces two new photoswitchable reagents for in vivo control of microtubule dynamics and related processes, showing their effectiveness in 3D systems and model organisms.

Lattice defects induced by microtubule-stabilizing agents exert a long-range effect on microtubule growth by promoting catastrophes

This study shows that microtubule-stabilizing agents induce lattice defects that cause long-range catastrophes in microtubule growth.

Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx)

This paper introduces TREx, a modified expansion microscopy method that achieves ten-fold sample expansion without specialized equipment, enabling high-resolution imaging of both thick tissue sections and cultured cells.

Kinesin-4 KIF21B limits microtubule growth to allow rapid centrosome polarization in T cells.

This study shows that KIF21B limits microtubule growth to enable rapid centrosome polarization in T cells by inducing microtubule pausing and catastrophe.

Talin Rod Domain Containing Protein 1 (TLNRD1) is a novel actin- bundling protein which promotes filopodia formation

This study identifies TLNRD1 as an actin-bundling protein that promotes filopodia formation and cell migration by interacting with LD-motif proteins like RIAM and KANK.

Concerted action of kinesins KIF5B and KIF13B promotes efficient secretory vesicle transport to microtubule plus ends

The paper shows that KIF5B and KIF13B work together to transport secretory vesicles to microtubule plus ends, with KIF5B playing a dominant role and KIF13B assisting in reaching newly polymerized ends.

Two antagonistic microtubule targeting drugs act synergistically to kill cancer cells

The paper identifies Carba1, a microtubule-destabilizing drug, which synergizes with paclitaxel to enhance cancer cell killing in vitro and in vivo.

Pharmaceutical-grade Rigosertib is a Microtubule-destabilizing Agent

This paper shows pharmaceutical-grade rigosertib, with high purity, causes microtubule destabilization in cells, confirming its mechanism of action against cancer cells.

CLASP mediates microtubule repair by promoting tubulin incorporation into damaged lattices

This study shows CLASP2 promotes microtubule lattice repair by facilitating tubulin incorporation into damaged sites and stabilizing partial protofilaments.

Mechanisms of motor-independent membrane remodeling driven by dynamic microtubules

This study reconstitutes membrane tubulation using dynamic microtubules and proteins, revealing three motor-independent mechanisms for membrane remodeling.

Photoswitchable microtubule inhibitors enabling robust, GFP-orthogonal optical control over the tubulin cytoskeleton

The paper introduces photoswitchable microtubule inhibitors that enable optical control over microtubule dynamics using a novel scaffold with improved chemical and biochemical properties.

Structural determinants of microtubule minus end preference in CAMSAP CKK domains

This study compares human and Naegleria CKK domains to show how structural differences in tubulin conformations enable microtubule minus-end recognition.

MAP7 family proteins regulate kinesin-1 recruitment and activation

The paper shows that MAP7 family proteins activate kinesin-1 by enhancing its microtubule binding and processivity through transient interactions.

Optogenetic dissection of mitotic spindle positioning in vivo

This study uses optogenetics to dissect mitotic spindle positioning in C. elegans embryos, showing that G-GTP signaling is dispensable and LIN-5Mud/NuMA activates dynein-dependent forces.

Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots

This study uses quantum dots to investigate how cytoskeletal structures affect intracellular transport dynamics, revealing actin's role in suppressing microtubule remodeling and influencing diffusion rates.