GenomicsbioRxivPreprint
Guide RNA binding induces reverse transcription bias during RT-qPCR analysis of RNA-targeting CRISPR/Cas systems
Boyu Huang, Carlos Orosco, Elijah Stewart…·Piyush Jain
The paper shows guide RNA binding causes reverse transcription bias in RT-qPCR for CRISPR systems and proposes a denaturation step to correct it.
DOI 10.64898/2026.08.16.745121