Carlos Orosco

The paper presents the cryo-EM structure of AsCas12a bound to a pseudo-DNA guide and RNA target, revealing its mechanism of DNA-guided RNA recognition.

The paper shows guide RNA binding causes reverse transcription bias in RT-qPCR for CRISPR systems and proposes a denaturation step to correct it.

TAMIPAMI identifies PAM/TAM motifs in CRISPR and OMEGA systems using a single control and treated library, providing an accessible computational framework with accurate motif recovery.

This study introduces {Psi}DNA, a DNA-based guide for Cas12 enzymes that enables specific and efficient RNA targeting, achieving 100% accuracy in detecting HCV RNA.

SCOUR predicts metabolite-dependent regulatory interactions in metabolic networks using a stepwise machine learning framework, achieving high accuracy in identifying single-metabolite-controlled reactions.