Piyush Jain

This study investigates how TIGR-Tas systems recognize and cleave DNA targets, revealing asymmetric target engagement and coordinated cleavage across both RNA-DNA heteroduplexes.

The paper presents the cryo-EM structure of AsCas12a bound to a pseudo-DNA guide and RNA target, revealing its mechanism of DNA-guided RNA recognition.

The paper shows guide RNA binding causes reverse transcription bias in RT-qPCR for CRISPR systems and proposes a denaturation step to correct it.

TAMIPAMI identifies PAM/TAM motifs in CRISPR and OMEGA systems using a single control and treated library, providing an accessible computational framework with accurate motif recovery.

This study profiles Acomys cahirinus fibroblasts to show that Col3A1 expression and senescence-associated cell states influence matrix remodeling, contributing to scarless regeneration.

This paper introduces CODEMax and CODEMax(exo+) genome editing systems that enable efficient and precise search-and-replace editing in human cells with reduced off-target effects.

This study uses SAC-seq to map m6A modifications at single-nucleotide resolution in bovine preimplantation embryos, identifying a critical m6A site in RPL12 that regulates translation and development.

This study introduces {Psi}DNA, a DNA-based guide for Cas12 enzymes that enables specific and efficient RNA targeting, achieving 100% accuracy in detecting HCV RNA.

This paper develops a CRISPR-based diagnostic method using CO1 gene targeting to detect invasive moth pests with high accuracy and sensitivity.

This study identifies genetic loci and HLA associations linked to COVID-19 susceptibility in a Florida population, highlighting ancestral differences in disease risk.

The paper identifies HLA-DPA1 alleles associated with risk and protection from SARS-CoV-2 infection and COVID-19 severity through genotyping and structural modeling.