Boyu Huang

This study investigates how TIGR-Tas systems recognize and cleave DNA targets, revealing asymmetric target engagement and coordinated cleavage across both RNA-DNA heteroduplexes.

The paper presents the cryo-EM structure of AsCas12a bound to a pseudo-DNA guide and RNA target, revealing its mechanism of DNA-guided RNA recognition.

The paper shows guide RNA binding causes reverse transcription bias in RT-qPCR for CRISPR systems and proposes a denaturation step to correct it.

This study introduces {Psi}DNA, a DNA-based guide for Cas12 enzymes that enables specific and efficient RNA targeting, achieving 100% accuracy in detecting HCV RNA.