Guide RNA binding induces reverse transcription bias during RT-qPCR analysis of RNA-targeting CRISPR/Cas systems
Boyu Huang, Carlos Orosco, Elijah Stewart, Mahika Balaraju, Yasmin B. Elhabashy, Piyush Jain
The paper shows guide RNA binding causes reverse transcription bias in RT-qPCR for CRISPR systems and proposes a denaturation step to correct it.
bioRxiv · Aug 19, 2026 · PreprintGenomics