Streamlined, recombinase-free genome editing with CRISPR-Cas9 in Lactobacillus plantarum reveals barriers to efficient editing
Ryan T. Leenay, Justin M. Vento, Malay Shah, Maria Elena Martino, François Leulier, Chase L. Beisel
This study introduces a CRISPR-Cas9-based genome editing method for Lactobacillus plantarum without requiring recombinase, showing it can efficiently introduce stop codons and deletions but with some unexpected outcomes.
bioRxiv · Jun 20, 2018 · PreprintSynthetic Biology