Author

Ryan T. Leenay

North Carolina State University

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Synthetic Biology· 2 Cell Biology· 1

Signature Assays, Organisms & Methods

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Organism:bacteria (other)· 2 Approach:experimental assay· 2 Assay:whole-genome sequencing· 2 Assay:CRISPR screen· 1 Research Focus:drug response / drug discovery· 1 Organism:human· 1 Approach:machine learning (classical)· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

Chase L. BeiseliD· 2 shared Albert J. KeungiD· 1 shared Alexander MarsoniD· 1 shared Amirali Aghazadeh· 1 shared Andrew P. MayiD· 1 shared Chunyu LiaoiD· 1 shared David Tse· 1 shared Fani Ttofali· 1 shared

Activity by Year

2018-2018 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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2 shared papersSynthetic Biology

Chase L. BeiselORCID

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Author profile →

3 papers

Recent·Most cited

Systematic characterization of genome editing in primary T cells reveals proximal genomic insertions and enables machine learning prediction of CRISPR-Cas9 DNA repair outcomes

Ryan T. Leenay, Amirali Aghazadeh, Joseph Hiatt, David Tse, Judd F. Hultquist, Nevan Krogan, Zhenqin Wu, Alexander Marson, Andrew P. May, James Zou

This study systematically characterizes genome editing in primary T cells, identifies proximal genomic insertions, and develops a machine learning model to predict CRISPR-Cas9 DNA repair outcomes.

bioRxiv · Aug 31, 2018 · PreprintCell Biology

Streamlined, recombinase-free genome editing with CRISPR-Cas9 in Lactobacillus plantarum reveals barriers to efficient editing

Ryan T. Leenay, Justin M. Vento, Malay Shah, Maria Elena Martino, François Leulier, Chase L. Beisel

This study introduces a CRISPR-Cas9-based genome editing method for Lactobacillus plantarum without requiring recombinase, showing it can efficiently introduce stop codons and deletions but with some unexpected outcomes.

bioRxiv · Jun 20, 2018 · PreprintSynthetic Biology

One-step assembly of large CRISPR arrays enables multi-functional targeting and reveals constraints on array design

Chunyu Liao, Fani Ttofali, Rebecca Slotkowski, Steven R. Denny, Taylor D. Cecil, Ryan T. Leenay, Albert J. Keung, Chase L. Beisel

The paper introduces CRATES, a one-step method to assemble large CRISPR arrays, demonstrating its utility for multiplexed gene editing and revealing design constraints.

bioRxiv · May 2, 2018 · PreprintSynthetic Biology