Chase L. Beisel

The paper shows that cleaved aminoacylated tRNA tails trigger auxiliary bacterial immunity by activating Adt1, leading to mRNA cleavage and cellular dormancy.

This paper describes how the Druantia type III-A defense system in E. coli clears phage infections by recognizing single-stranded DNA and unwinding DNA duplexes directionally.

The paper identifies structured RNA motifs and Aca proteins that regulate anti-CRISPR gene expression in bacteriophages, revealing new regulatory mechanisms beyond DNA-binding HTH regulators.

This paper demonstrates that reprogrammed tracrRNAs can enable RNA base editing and trans-splicing through Cas9 recruitment, offering a versatile method for RNA manipulation.

This paper introduces a method for efficient, single-step genome editing of bacteriophages using targeted DNA nicking, achieving high recombination rates without reducing phage titers.

The paper identifies YprA family helicases as a missing link connecting DISARM and Druantia immune systems in prokaryotes, revealing new defense classes and their antiviral mechanisms.

This paper identifies viral single-guide RNAs (vsgRNAs) that subvert bacterial CRISPR-Cas9 immunity by sequestering Cas9 and redirecting its activity, and shows they can enable genome editing in mammalian cells.

This study shows that Cas12a2 preferentially cleaves collateral DNA over RNA, revealing the mechanism and implications for CRISPR-based immunity and technology.

The paper develops a base-editing pipeline for multi-gene knockouts in Salmonella Typhimurium, showing reduced virulence but also off-target mutations affecting fitness.

This study characterizes a type II Thoeris system in E. coli that causes growth arrest without NAD+ depletion, revealing functional diversity within Thoeris types.

The paper analyzes CRISPR array repeat mutations across prokaryotic genomes, revealing subtype-specific patterns and a link between repeat mutations and spacer dynamics.

This study optimizes a genome editing system for Cupriavidus necator H16, achieving high editing efficiency using an improved SIBR-Cas system.

This paper shows AcrVIB1 inhibits CRISPR-Cas13b by promoting unproductive crRNA binding and degradation, revealing a mechanism to suppress immune defense.

This paper introduces append editing, a method that uses ADP-ribosylation to achieve distinct genome editing outcomes in bacteria and eukaryotes.

IMPRINT uses cell-free transcription-translation to methylate DNA, bypassing bacterial restriction and improving transformation efficiency in diverse bacteria.

This paper shows that reducing DNA targeting activity in CRISPR systems improves genome editing efficiency in bacteria by enabling RecA-mediated repair.

The paper develops a mixed-effect random forest model to predict CRISPRi guide efficiency using data from depletion screens and gene-specific features, outperforming existing methods.

This paper uses cell-free transcription-translation to rapidly characterize multi-subunit CRISPR effectors and transposons, revealing new insights into their targeting rules and transposition mechanisms.

This paper develops exonuclease-deficient cell-free extracts and optimized buffers to enable robust expression from unprotected linear DNA without additional protection strategies.

The paper shows that CRISPR-Cas13 systems require target transcript expression above a threshold to induce dormancy, distinguishing pathogenic from benign invaders and preventing autoimmunity.

This paper characterizes genetic elements in Saccharomyces boulardii to enable engineered biomolecule synthesis and delivery in the gut, showing tunable gene expression and colonization dynamics.

The paper characterizes two RNA regulators with distinct timescales and demonstrates their use in creating a genetic pulse generator through computational modeling and experimental validation.

This study introduces a CRISPR-Cas9-based genome editing method for Lactobacillus plantarum without requiring recombinase, showing it can efficiently introduce stop codons and deletions but with some unexpected outcomes.

The paper introduces CRATES, a one-step method to assemble large CRISPR arrays, demonstrating its utility for multiplexed gene editing and revealing design constraints.

This paper introduces an E. coli cell-free system to rapidly and scalably characterize CRISPR technologies, including DNA cleavage, gene repression, and anti-CRISPR activity.