A combined human in silico and CRISPR/Cas9-mediated in vivo zebrafish based approach for supporting gene target validation in early drug discovery
Matthew J. Winter, Yosuke Ono, Jonathan S. Ball, Anna Walentinsson, Erik Michaëlsson, Anna Tochwin, Steffen Scholpp, Charles R. Tyler, Steve Rees, Malcolm J. Hetheridge, Mohammad Bohlooly‐Y
The paper identifies three genes associated with heart failure using human exome sequencing and validates their roles in cardiovascular function using zebrafish CRISPR/Cas9 mutagenesis.
Frontiers in Pharmacology · Apr 25, 2022Peer-reviewedMolecular Biology