In-gel protein digestion using acidic methanol produces a highly selective methylation of glutamic 1 acid residues.
Marta Lozano-Prieto, Emilio Camafeita, Inmaculada Jorge, Andrea Laguillo-Gómez, Rafael Barrero-Rodríguez, Cristina A. Devesa, Clara Pertusa, Enrique Calvo, Francisco Sánchez‐Madrid, Jesús Vázquez, Noa Beatriz Martin-Cofreces
The paper shows that in-gel protein digestion with acidic methanol selectively methylates glutamic acid residues, not other methylable residues like aspartic acid.
Journal of Proteomics · Jun 14, 2024Peer-reviewedSystems Biology