BioengineeringbioRxivPreprint
Selection-free, high frequency genome editing by homologous recombination of human pluripotent stem cells using Cas9 RNP and AAV6
Renata M. Martin, Kazuya Ikeda, Nobuko Uchida…·Matthew Porteus
This paper introduces a Cas9/AAV6 system for high-frequency genome editing in human pluripotent stem cells without selection markers, achieving up to 94% integration efficiency for large DNA cassettes.
DOI 10.1101/252163