Hiromitsu Nakauchi

Quantum Barcoding Bridges High-Dimensional Immune Phenotyping with Signaling Responses across Species

The paper benchmarks Quantum Barcoding against mass cytometry in human and rhesus macaque blood, integrating data to reveal conserved and divergent signaling responses across species.

TorPause: Efficient Static Organ Preservation for Transplants

TorPause is a two-component preservation method that reduces ischemia-reperfusion injury in rat liver transplants by pausing metabolism and protecting mitochondria.

Donor-Targeted Anti-HLA-A2 Antibody Shows Nanogram-Level Efficacy in a Novel GVHD Mouse Model

This paper develops an HLA-A2-specific antibody that effectively reduces GVHD in a mouse model with minimal dose.

Hoxb5 Enriches Long-Term Hematopoietic Stem Cell Activity within the Mouse Fetal Liver Phenotypic HSC Compartment

This study identifies Hoxb5 as a marker for long-term hematopoietic stem cells in fetal liver, showing its utility in purification and revealing developmental regulation.

Beta-Adrenergic Stimulation and MYH7 G256E Mutant Gene Dosage Drive Hypertrophic Cardiomyopathy Phenotype Penetrance

This study investigates factors influencing HCM phenotype penetrance by comparing MYH7 mutations in isogenic hiPSC-CMs and shows that beta-adrenergic stimulation and gene dosage affect disease expression.

Xenophagocytosis blockade enhances interspecies chimerism

This study identifies xenophagocytosis as an innate immune barrier that limits interspecies chimerism and shows that blocking it enhances donor cell engraftment in mouse embryos.

DNMT3AR882H Is Not Required for Disease Maintenance in Primary Human AML, but Is Associated With Increased Leukemia Stem Cell Frequency

This study shows DNMT3AR882H mutations are not required for maintaining AML but are linked to higher leukemia stem cell frequency.

A human-specific regulatory mechanism revealed in a preimplantation model

The paper investigates the role of HERVK LTR5Hs elements in human preimplantation development, showing their cis-regulatory impact on the epiblast transcriptome and their essential role in blastoid formation through ZNF729 activation.

The generation of viable, structurally integrated human-mouse chimaeras through enhanced hPSCs proliferation

This paper enhances human pluripotent stem cell (hPSC) proliferation to improve chimaerism in human-mouse chimaeras, resulting in viable pups with integrated hPSC-derived tissues.

Development of iPSC-Derived T Cells Targeting EGFR Neoantigens in Non-Small Cell Lung Cancer

This study develops iPSC-derived T cells targeting the EGFR C797S mutation in NSCLC, showing their ability to recognize and kill cancer cells expressing this mutation.

Disentangling cell-intrinsic and extrinsic factors underlying evolution

The paper decomposes evolutionary divergence into cell-intrinsic, extrinsic, and interaction components using reciprocal rat-mouse chimeras and finds cell-intrinsic factors dominate but extrinsic factors also play a role.

Stable platelet production via the bypass pathway explains the long-term reconstitution capacity of hematopoietic stem cells

This study identifies a myeloid bypass pathway that supports long-term HSC reconstitution by analyzing single-cell transplantation data with a mathematical model.

Generation of iPSC-derived CD4+ Th1 cells enhancing chimeric antigen receptor-T cell cytotoxicity

This study generates CD4+ Th1 cells from iPSCs to enhance CD8+ CAR-T cell cytotoxicity against GD2-expressing lymphoma.

Inter-cellular mRNA Transfer Alters Human Pluripotent Stem Cell State

The paper shows that inter-species mRNA transfer from mouse embryonic stem cells to human pluripotent stem cells reprograms them to a naive-like state through specific transcription factors.

Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival

This study transplants human iPSC-derived pancreatic progenitors into Pdx1-deficient mice, extending their lifespan and demonstrating functional engraftment of human pancreatic cells.

Secreted Particle Information Transfer (SPIT) - A Cellular Platform For In Vivo Genetic Engineering

The paper introduces SPIT, a method using human cells to deliver genetic tools in vivo, demonstrating its application for modifying multiple organs and tissues in immunocompetent mice.

In vivo CRISPR screening identifies SAGA complex members as key regulators of hematopoiesis

This study uses in vivo CRISPR screening to identify SAGA complex members as key regulators of hematopoiesis, showing their loss impairs blood production and activates interferon pathways.

Cas9-AAV6 Gene Correction of Beta-Globin in Autologous HSCs Improves Sickle Cell Disease Erythropoiesis in Mice

This study corrects the beta-globin gene in sickle cell disease patient-derived HSCs using CRISPR/Cas9 and shows improved in vivo erythropoiesis in a mouse model.

Streamlined and quantitative detection of chimerism in mouse tissue using digital PCR

This paper develops a droplet digital PCR assay to quantify chimerism in mouse tissues, showing it is robust, minimally biased, and compatible with crude lysates.

Cross-species blastocyst chimerism between nonhuman primates using iPSCs

This paper demonstrates that chimpanzee iPSCs can form cross-species chimeras with rhesus macaque embryos, suggesting functional similarity between human and chimpanzee cells.

Selection-free, high frequency genome editing by homologous recombination of human pluripotent stem cells using Cas9 RNP and AAV6

This paper introduces a Cas9/AAV6 system for high-frequency genome editing in human pluripotent stem cells without selection markers, achieving up to 94% integration efficiency for large DNA cassettes.

Establishment In Culture Of Expanded Potential Stem Cells

This paper establishes expanded potential stem cells (EPSCs) from mouse blastomeres and shows they can contribute to both embryo proper and trophoblasts in chimeras.