Single-cell quantification of the microbiota by flow cytometry: MicFLY
Christine M. Tin, Bianca Cordazzo Vargas, Darryl Adelaide Abbott, Aditi R. Lohar, Tiffany C. Taylor, Ivan A. Valishev, Sarah N. Weinshel, Vung Lian, Kacey J. Sullinger, Matthew Butoryak, Michael A. Silverman, Liat Shenhav, William H. DePas, Timothy Wesley Hand
The paper develops MicFLY, a flow cytometry method to quantify and characterize intestinal microbiota at the single-cell level, revealing E. coli unbound by IgG and IgA is associated with necrotizing enterocolitis.
bioRxiv · Sep 18, 2025 · PreprintMicrobiology