Isotope-Free Mapping of protein:RNA Interactions Using fCRAC and trxtools
Nic Robertson, Jan Mikolajczyk, Andrea Cecilia Garcia-Sandoval, Aleksandra Helwak, Maxeen L Major, Anouk Emadali, David W. Tollervey, Tomasz W. Turowski
The paper introduces fCRAC, a method for high-resolution mapping of protein:RNA interactions without radioactive labeling, and provides trxtools for analysis, revealing interactions of CYCLON/CCDC86 with rRNA, tRNA, and ncRNAs.
bioRxiv · May 21, 2026 · PreprintMolecular Biology