Tomasz W. Turowski

Isotope-Free Mapping of protein:RNA Interactions Using fCRAC and trxtools

The paper introduces fCRAC, a method for high-resolution mapping of protein:RNA interactions without radioactive labeling, and provides trxtools for analysis, revealing interactions of CYCLON/CCDC86 with rRNA, tRNA, and ncRNAs.

CYCLON is a nucleolar protein that regulates pan-cancer cell fitness through ribosome biogenesis

This study identifies CYCLON as a nucleolar protein that regulates cancer cell fitness by controlling ribosome biogenesis and nucleolar structure across multiple tumor types.

High-resolution mapping of human RNA polymerase III reveals transcription termination as a rate-limiting step

The paper maps human RNA polymerase III transcription dynamics, finding that termination is the rate-limiting step for highly expressed tRNA genes.

Transcription termination by RNA polymerase I

The paper investigates transcription termination by RNA polymerase I, showing that 3-end cleavage and backtracking promote termination, with biophysical modeling suggesting ~10% premature termination.

Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast

A de novo yeast neochromosome carrying all 275 tRNA genes was built and characterized; it imposes a growth burden, with genome doubling and deletions in haploids possibly reflecting compensation.

Nascent transcript folding plays a major role in determining RNA polymerase elongation rates

The paper investigates how nascent transcript folding and G+C-content influence RNA polymerase elongation rates in vivo and confirms these effects through in vitro experiments and a mathematical model.