An orthogonal and pH-tunable sensor-selector for muconic acid biosynthesis in yeast
Tim Snoek, David Romero-Suárez, Jie Zhang, Francesca Ambri, Mette L. Skjoedt, Suresh Sudarsan, Michael Krogh Jensen, Jay D. Keasling
The paper develops a synthetic selection system in yeast to enrich for high-producing muconic acid variants by coupling production to cell fitness through a pH-tunable sensor-selector.
ACS Synthetic Biology · Apr 3, 2018Peer-reviewedSynthetic Biology